Princess Twilight Sparkle (both
regular and
fashion size) and
Sunset Shimmer from the upcoming Through The Mirror line have gradient eyes, and the printing has been modified to put eye shadow and glitter on them, so it's definitely brand new but still with gradient.
Rarity from the Midnight in Canterlot collection doesn't have a very visible gradient, but everyone else from the same set do have gradients! Even
Fluttershy, who has a brand new "open" eye shape, and
Rainbow Dash, who has Cherry Berry's eyes, printed on her for the first time with round eye shines instead of the hexagonal gem shines that we've seen twice before.